A Preliminary Genetic Linkage Map of Sinonovacula constricta(Lamarck, 1818) Based on Microsatellites Derived from RAD Sequencing     

WU Xueping  FENG Yanwei  JIANG Hailin  LIU Xiangquan  PAN Ying 《中国海洋大学学报(英文版)》2018,(4)

Sinonovacula constricta is one of the important economic aquaculture species in China. In this study, we constructed genetic linkage maps of S. constricta based on 300 microsatellite markers derived from RAD-seq using an F1 full-sib family. The female map contained 204 microsatellites assigned to 22 linkage groups, which covered 1529.5 cM with an average interval of 10.3 cM. The male consisted of 187 microsatellites in 19 linkage groups corresponding to the haploid chromosome number(n(28)19), which spanned 1429.3 cM with an average interval of 8.7 cM. The genome coverage was approximately 83.5% and 81.4%, respectively. An integrated map was constructed according to the common markers in parental linkage groups, which had a total length of 1683.8 cM with an average interval of 7.3 cM. The genome coverage of the integrated map was approximately 86.3%. The genetic linkage map would form the foundation for further studies on the quantitative trait loci(QTL), as well as accelerating the breeding process of this species.  相似文献   

Levels of Metabolic Enzymes and Nitrogenous Compounds in the Swimming Crab Portunus trituberculatus Exposed to Elevated Ambient Ammonia-N     

PAN Luqing  SI Lingjun  LIU Shengnan  LIU Maoqi  WANG Guohui 《中国海洋大学学报(英文版)》2018,(4)

This study investigated the effects of ammonia-N(control and 1 and 5 mg L~(-1)) on the changes of metabolic enzymes activities and nitrogenous compounds levels in the gills, hepatopancreas, muscles, and hemolymph of the swimming crab Portunus trituberculatus. Results showed that the levels of hemolymph ammonia increased significantly at 6 h and then decreased to control level at 12 h. The activities of key enzymes involved in biosynthesis pathways of glutamine(Gln) and urea, such as glutamate dehydrogenase(GDH), glutamine synthetase(GS), and arginase(ARG), increased remarkably in different tissues of P. trituberculatus. Gln level was the highest in muscles, while urea content was the largest in hepatopancreas. The m RNA expression level of xanthine dehydrogenase(XDH) was downregulated sharply in gills but upregulated in the hepatopancreas and muscles. A small amount of uric acid was detected in the hemolymph. This result suggests that Gln in muscles may be used as an intermediary nitrogen repository, while urea may be primarily synthesized in the hepatopancreas and then transferred to other tissues via the hemolymph. Uric acid could be synthesized by the anabolism of purine nucleotides, in additon to the inhibitory activities of gills. In addition, the levels of Gln, urea and uric acid exhibited dose-and time-dependent effects with ammonia content. Therefore, P. trituberculatus could possibly convert ammonia into Gln, urea, or uric acid to detoxify ammonia during high ambient ammonia-N stress.  相似文献   

Cryosphere evapotranspiration in the Tibetan Plateau: A review     

KunXin Wang  YinSheng Zhang  Ning Ma  YanHong Guo  YaoHui Qiang 《寒旱区科学》2020,12(6):355-370

Land surface actual evapotranspiration is an important process that influences the Earth's energy and water cycles and determines the water and heat transfer in the soil-vegetation-atmosphere system. Meanwhile, the cryosphere's hydrological process is receiving extensive attention, and its water problem needs to be understood from multiple perspectives. As the main part of the Chinese cryosphere, the Tibetan Plateau faces significant climate and environmental change. There are active interaction and pronounced feedback between the environment and ET_a in the cryosphere. This article mainly focuses on the research progress of ET_a in the Tibetan Plateau. It first reviews the ET_a process, characteristics, and impact factors of typical underlying surfaces in the Tibetan Plateau (alpine meadows, alpine steppes, alpine wetlands, alpine forests, lakes). Then it compares the temporal and spatial variations of ET_a at different scales. In addition, considering the current greening of cryosphere vegetation due to climate change, it discusses the relationship between vegetation greening and transpiration to help clarify how vegetation activities are related to the regional water cycle and surface energy budget.  相似文献   

南沙他国侵占岛礁战略价值及空间格局评价     

闵开付  程亮  周晓  夏南  李宁  李满春 《热带地理》2020,40(4):684-693

从建岛潜力、岛礁辐射能力和岛礁承载能力3个方面选取26个指标，采用主客观综合赋权法确定各因子权重，利用线性加权法获得被越南、菲律宾、马来西亚、文莱等国所侵占的南沙44个岛礁的战略价值，并对结果进行空间插值，分析其空间格局特征。结果表明：1）被马来西亚侵占的弹丸礁、越南侵占的南威岛、以及菲律宾侵占的中业岛，其战略价值位列前3，评价得分分别为100、98.42和97.09。2）岛礁辐射能力空间分布格局呈现“NW—SE条带式”，“最高”和“高”等级的区域由研究区西北延伸至东南，然后向东北、西南两侧递减，辐射能力格局中有2个核心区域和2个次级核心区域。承载能力空间分布格局呈现“横向条带式”，由北向南越来越低，有1个核心区域和2个次级核心区域。建岛潜力空间分布格局有3个核心区域及2个次级核心区域。3）岛礁战略价值空间分布格局有3个核心区域，分布于研究区中部、北部及南部，与越南、菲律宾、马来西亚密切相关的核心区域各有1个。越南侵占岛礁最多且空间分布较广，有2个次级核心区域也与越南密切相关，越占岛礁若进一步建设，将形成多核心协同的战略格局，需密切关注。  相似文献   

地缘政治风险研究进展与展望     

熊琛然  王礼茂  屈秋实  向宁  王博 《地理科学进展》2020,39(4):695-706

地缘政治风险是影响全球和区域和平、稳定与发展的5大风险之一。如何识别、评估、预测和管理地缘政治风险成为国内外共同关注的问题。国内外学者对地缘政治风险这一术语的定义还不统一。当前地缘政治风险研究,在致险因素分析、风险影响刻画、风险量化与制图等领域都有一些新进展。针对地缘政治风险致险因子的时空差异性和多变性、各致险因子相互影响与反馈机制的复杂性以及地缘政治风险的突发性和不确定性,对致险因素的精准识别,地缘政治风险形成的机理,地缘政治风险监测与模拟等研究成为核心和前沿问题。中国未来地缘政治风险和重要研究方向包括：周边地区地缘政治风险研究;地缘政治风险的定量化与模拟研究;跨学科、大数据、多终端的地缘政治风险集成计算方法与预警服务平台建设;凝炼科学问题,提升对现实地缘政治问题的解释能力。  相似文献   

机载激光雷达测深技术研究与进展     

王鑫  潘华志  罗胜  杨鑫丽 《海洋测绘》2019,39(5):78-82

当前包括内河、水库及海域在内的水上、水下地形测量任务需求不断增加,进行水域或海洋测绘领域研究已提升到新的国家战略性高度。首先介绍了当前机载激光雷达水深测量的基本原理;概括总结了激光测深中的波形处理、波浪潮汐改正、折射改正、航带拼接与数据融合等关键技术,对测深的精度及误差影响进行了分析。最后结合当前技术的发展及新技术的出现,对未来机载激光雷达测深技术的发展进行了展望。  相似文献   

逆时偏移在声反射成像测井中的应用方法研究          下载免费PDF全文

李雨生  李宁  武宏亮  王克文  冯周  刘鹏 《地球物理学报》2020,63(4):1705-1714

声反射成像测井中常用的基于射线理论的Kirchhoff积分偏移算法和基于单程波理论的F-K偏移算法均可实现井旁缝洞反射体的快速偏移成像,但其仅适用于地层垂向变化较弱的速度场和高陡角裂缝的偏移成像,无法实现低角度反射体的准确偏移归位,产生偏移假象误导测井解释.逆时偏移基于全波动方程,可适应强垂向变化速度场,实现近似水平反射体的偏移成像.本文详细分析了将逆时偏移应用于声反射成像测井时存在的数据准备、时间采样间隔匹配和成像条件改进等若干问题,通过设置多组理论模型来说明算法对井旁不同反射体的识别能力.模拟资料和实际资料处理结果证实,较F-K偏移算法,逆时偏移算法成像精度更高、收敛性更好,可有效实现近似水平构造偏移归位.改进的归一化互相关成像条件可解决深部地层的远井壁成像衰减问题,降低测井解释的多解性.逆时偏移将成为声反射成像测井高精度偏移技术的发展方向.  相似文献   

Watershed-scale vegetation,water quantity,and water quality responses to wildfire in the southern Appalachian mountain region,United States     

Peter V. Caldwell  Katherine J. Elliott  Ning Liu  James M. Vose  David R. Zietlow  Jennifer D. Knoepp 《水文研究》2020,34(26):5188-5209

Wildfires are landscape scale disturbances that can significantly affect hydrologic processes such as runoff generation and sediment and nutrient transport to streams. In Fall 2016, multiple large drought-related wildfires burned forests across the southern Appalachian Mountains. Immediately after the fires, we identified and instrumented eight 28.4–344 ha watersheds (four burned and four unburned) to measure vegetation, soil, water quantity, and water quality responses over the following two years. Within burned watersheds, plots varied in burn severity with up to 100% tree mortality and soil O-horizon loss. Watershed scale high burn severity extent ranged from 5% to 65% of total watershed area. Water quantity and quality responses among burned watersheds were closely related to the high burn severity extent. Total water yield (Q) was up to 39% greater in burned watersheds than unburned reference watersheds. Total suspended solids (TSS) concentration during storm events were up to 168 times greater in samples collected from the most severely burned watershed than from a corresponding unburned reference watershed, suggesting that there was elevated risk of localized erosion and sedimentation of streams. NO₃-N concentration, export, and concentration dependence on streamflow were greater in burned watersheds and increased with increasing high burn severity extent. Mean NO₃-N concentration in the most severely burned watershed increased from 0.087 mg L⁻¹ in the first year to 0.363 mg L⁻¹ (+317%) in the second year. These results suggest that the 2016 wildfires degraded forest condition, increased Q, and had negative effects on water quality particularly during storm events.  相似文献   

基于GPS应变、地震应变率与震源应力场对帕米尔高原现今构造变形特征的分析          下载免费PDF全文

潘正洋  邵志刚  周云  王洵  解孟雨  艾萨·伊斯马伊力 《地球物理学报》2020,63(3):1143-1154

研究帕米尔高原的构造变形特征对于理解印度板块向北推挤过程中的应变分配方式以及应力转换模式具有重要的意义.本文利用区域GPS应变场、地震应变场与震源应力场分析帕米尔高原的构造形变特征.主要结论为:(1)该区域变形主要以NNW-SSE或近N-S向的挤压为主,在高原内部伴有明显的近ENE-WSW或E-W向拉张,应力方向在帕米尔高原与塔吉克盆地区域呈现逆时针旋转的趋势,而在塔里木盆地则显示几乎与帕米尔高原的一致的应力状态,这可能与两侧盆地块体的强度差异有关.(2)安德森断层参数A∅显示帕米尔高原北缘与西侧区域为逆断层应力状态,在高原内部为正断层应力状态,这与GPS应变的结果显示的应变主要集中在主帕米尔断裂与阿莱谷地附近而在高原内部应变较低是一致的,另外应力在喀喇昆仑断裂北段的方向基本平行于断层走向,以及断层北端较低的滑动速率,这说明了地壳挤压缩短可能是帕米尔高原主要的的构造变形特征,并不支持由于边界走滑断裂导致的应变分异或者块体挤出的模式.(3)综合考虑地震应变方向与SHmax从帕米尔北部NNW-SSE方向到天山北部的近N-S方向的转换,GPS应变方向在帕米尔高原两侧盆地都存在不同程度的旋转,应力场安德森参数A∅显示的应力状态以及SKS的结果显示的近ENE-WSW方向,我们认为印度板块向北推挤与天山造山带碰撞导致帕米尔高原不对称的径向逆冲是帕米尔高原现今构造变形的主要成因与构造模式.  相似文献   

基于修正SWAT模型的岩溶地区非点源污染模拟初探——以横港河流域为例   总被引：3，自引：0，他引：3  

赖格英  易姝琨  刘维  盛盈盈  彭小娟  熊家庆  潘思怡  吴青 《湖泊科学》2018,30(6):1560-1575

岩溶流域含水系统的主要特征之一是连通地表的落水洞等垂直管道将近水平的地下暗河联系起来，降水及其形成的地表径流可以通过这些管道迅速地灌入地下河系，从而改变了水及其所携带的非点源污染物质在垂直与水平方向的传输速度与数量，使岩溶流域内地表-地下之间的物质交换与传输过程变得比较复杂；应用广泛的SWAT模型在模拟岩溶地区的水文、水质时会存在一些不足与局限.为此，本文针对岩溶水系统特征，引入落水洞、伏流、暗河的水文过程以及主要营养盐的输移过程，修正SWAT模型原有的水文循环过程及相关算法，改变其只适用于松散均匀介质流域非点源污染模拟的单一特征，并研究建立适合于岩溶流域的非点源污染模型和相应的模拟方法.选取横港河流域岩溶地区作为非点源污染的对象，应用修正后的模型通过控制性的模拟方法和敏感性性分析，定量评估落水洞、伏流、暗河等岩溶特征对氮、磷等主要非点源污染物质输移的影响及其带来的时空效应，并进一步探讨落水洞、伏流、暗河等对地表-地下水文与营养盐的交互作用及转换机理.结果表明，岩溶特征对流域的氮、磷负荷有增加作用，其中总磷的增加明显大于总氮的增加，总磷和总氮的增量分别为0.86%和2.12%；植被岩溶指数的增加会导致流域可溶性磷、有机磷的产出量增加，有机氮、地表产流中硝酸氮和沉积磷的产出量则居其次，落水洞改变了降雨的产流方式，增加了落水洞所在流域的有机磷和有机氮的产出，其增量变化在0~0.7和0~0.3 kg/hm²之间.  相似文献